CAMLPR Microbiology Practice Test

These questions are part of the practice quiz. Start practicing

• Which Haemophilus species lacks fluorescence of ALA-porphyrins in ALA-porphyrin testing?
• Which organisms are commonly considered normal urinary tract flora?
• Which organism is non-lactose fermenting, oxidase negative, nitrate negative, and non-motile?
• Which media are used for anaerobic specimen recovery?
• Which medium is used to help recover Yersinia enterocolitica from stool cultures?
• Which medium is used to grow Corynebacterium diphtheriae and yields black-gray colonies?
• Which organism is a Gram-positive coccus in chains and is PYR positive and typically non-hemolytic on blood agar?
• Which of the following is NOT a common reason for false negatives in PCR testing?
• If an anaerobic specimen from suspected food poisoning shows terminal GPB spores and swarming on an ANO2 BAP plate, which organism is suspected?
• A Gram-positive coccus in chains that is PYR positive and lacks hemolysis on blood agar points to which genus?
• Which organism is a gram-negative cocci, oxidase positive, that ferments glucose, maltose, and sucrose but not lactose?
• Gram-positive cocci in clusters, catalase positive, tube coagulase positive. Which organism matches this profile?
• A 45 year old male presents with jaw swelling and pain around a recently extracted tooth. There is drainage around the incision and a minor fever. An aspirate from the abscess was cultured. The culture shows white-gray molar-tooth-like colonies on blood agar by day 3; Brucella agar also shows small similar colonies; thioglycolate broth appears turbid; Gram stain shows Gram-positive branching filaments with sulfur granules; Penicillin is susceptible. Which organism is suspected?
• A Gram-positive bacillus with boxcar arrangement, reverse CAMP positive and double-zone beta-hemolysis. Identify the organism.
• Which organism is a GNB with gull wing shape, oxidase positive, motile, and weak catalase positive?
• Chemotherapy patient presents with persistent cough and fever; BAL sample cultured. Sabouraud dextrose agar yields green mold; Gram stain shows fruiting bodies and separate branching hyphae. Which organism is suspected?
• A 3-year-old unvaccinated child presents with high fever, severe sore throat, drooling, and respiratory distress. The culture shows smooth translucent colonies on chocolate agar, no growth on blood agar, growth around Staphylococcus aureus on blood agar, slight oxidase activity, bubbling catalase, and red fluorescence for ALA-porphyrins is absent. Which organism is most likely?
• A 67-year-old hospitalized patient with fever and an indwelling catheter has been on prolonged antibiotic therapy. A urine culture shows Gram-negative rods. MAC pale; Blood agar shows brick-red colonies; Oxidase yellow; Motility motile; DNase halo; TSI K/A with no black precipitate. Which organism do you suspect?
• Which medium is used for fastidious organisms and requires CO2?
• Which sequence correctly explains a midstream urine collection to a patient?
• Which organism forms large white ground-glass colonies on blood agar and appears as boxcar-shaped Gram-positive bacilli that are non-motile?
• Which genital specimens are appropriate for culture to test for Neisseria gonorrhoeae?
• Which organism presents as Gram-negative diplococci with kidney bean shape and is associated with otitis media in adults, often testing positive for butyrate esterase?
• A 20-year-old patient presents with dysuria and suprapubic pain. A midstream urine sample is cultured. Gram stain shows Gram-positive cocci in clusters. Blood agar shows white opaque colonies. Catalase bubbling. Coagulase negative. Novobiocin: growth ~20 mm. Which organism do you suspect?
• Which specimen uses a yellow top SAF fixative container?
• What is the standard urinary CFU/L threshold for reporting significant bacteriuria?
• A Gram-negative rod that grows on BBE agar is most likely which genus?
• Which organism is non-lactose fermenting, oxidase negative, nitrate positive, VP positive, and motile?
• Which organism is typically treated with macrolides due to lack of cell wall?
• Which organism is non-lactose fermenting, oxidase negative, nitrate positive, PAD positive, citrate positive, and indole positive?
• Gram-positive cocci in chains, beta-hemolytic, Lancefield group B. Identify the organism.
• Under which scenario would you work up a urinary isolate?
• Which organism is lactose fermenting, Voges-Proskauer positive, motile, and lysine negative?
• Which medium are enriched?
• A 10 year old child presents with sudden sore throat, fever, painful swallowing, and tonsillar exudates with no cough. A throat swab was obtained. Gram stain shows Gram-positive cocci in chains; beta-hemolytic grey colonies; catalase negative; PYR positive; Lancefield grouping is A. What organism are you suspecting?
• What sputum would you accept?
• Which organism shows lecithinase activity on egg yolk agar?
• Which organism demonstrates porphyrin positivity?
• Which antibiotic class should be avoided when treating Enterococcus infections?
• Which wound specimen would be rejected for culture?
• A 29 year old patient presents with a diffuse rash and fever. A painless genital ulcer occurred weeks earlier. A skin lesion is cultured and fluorescent staining shows thin spiral spirochetes. What organism are you suspecting and what additional testing would you perform for confirmation?
• Which of the following is considered a priority specimen in microbiology?
• Which organism is a Gram-positive coccus in chains that is PYR positive and often described as non-hemolytic?
• Which Haemophilus species shows red fluorescence for ALA-porphyrins during testing?
• Gram-positive cocci in clusters, catalase positive, tube coagulase negative, novobiocin susceptible. Which organism is most likely?
• A CHROMagar screening grows pink colonies. What is the recommended next step?
• A 27-year-old pregnant woman at 36 weeks gestation is screened during prenatal care. A vaginal swab is cultured in Tod-Hewitt broth. Gram stain shows Gram-positive cocci in chains. Blood agar shows gray colonies with narrow beta-hemolysis. CAMP test shows arrow-shaped hemolysis near Staphylococcus aureus. Hippurate hydrolysis is blue. Which organism is suspected?
• Gram-positive cocci in clusters, catalase positive, tube coagulase negative, novobiocin resistant. Which organism is most likely?
• What type of test is commonly used for initial screening of syphilis?
• A 26-year-old patient presents with dysuria and cloudy urine. Urine culture shows Gram-negative rod, large gray colonies on BAP, pink on MAC, oxidase positive, TSI A/A, motile, citrate green, MUG positive, Indole positive (red). Which organism is most likely?
• Which organism is a Gram-positive coccus that occurs in chains, is non-hemolytic, and PYR positive?
• A 19-year-old patient presents with a persistent dry cough, headache, and sore throat. A nasopharyngeal swab is cultured. Gram stain shows no staining, and blood agar with sterols yields fried-egg-like colonies. Which organism is suspected and how would you confirm identification?
• Which organism is non-lactose fermenting, oxidase negative, nitrate positive, PAD positive, citrate negative, and indole positive?
• A 34-year-old technologist presents with mild fever and malaise, with significant edema and notable ulcer. A tissue sample was cultured. BAP shows large, white, ground-glass-like colonies; Gram stain shows boxcar Gram-positive bacilli; Motility is non-motile. Which organism is suspected?
• Which organism yields a positive Reverse CAMP test?
• A 28-year-old patient presents with bloody diarrhea; Campy culture conditions on microaerophilic environment show curved Gram-negative bacilli with gull-wing arrangement; Motility is darting; Oxidase positive; Catalase bubbles; Hippurate hydrolysis is positive. Which organism is suspected?
• Which organism is a gram-negative coccus, oxidase positive, and ferments glucose but not maltose, sucrose, or lactose?
• An elderly hospitalized patient with dysuria has a culture showing BAP smooth, non-hemolytic, opaque colonies; MAC colorless; CHOC growth enhanced; Gram stain shows short GNB resembling coccobacilli; oxidase negative, catalase positive (bubbles); motility non-motile; TSI K/K with no gas or H2S. Which organism is suspected?
• A catheterized elderly man with fever and dysuria has urine culture showing Gram-positive cocci in pairs, catalase negative, bile esculin positive with blackened colonies, and PYR pink. Which organism is suspected?
• Stenotrophomonas maltophilia is commonly treated with TMP-SMX or levofloxacin and is resistant to which class of antibiotics?
• Which description matches stool parasite/ova collection using a yellow top SAF fixative container?
• Beta-hemolytic streptococcus with Lancefield Group B. Which organism is it?
• For infections caused by vancomycin-resistant enterococci (VRE), which agent is indicated?
• Which set of media is used for stool cultures to detect a range of gastrointestinal organisms?
• For suspected Chlamydia trachomatis conjunctivitis, how should the specimen be collected and confirmed?
• Which agents are used to treat Enterococcus infections, including VRE considerations?
• 39-year-old hunter with fever, chills, headache, painful ulcer, and swollen lymph nodes. An ulcer swab is cultured. Gram stain shows tiny Gram-negative coccobacilli; CHOC supplemented with cysteine yields small smooth colonies; BAP non-supplemented and MAC are not growth; Oxidase positive; Catalase positive with small bubbling. Which organism is suspected?
• For respiratory specimens such as sputum or BAL, which combination of media and conditions is appropriate?
• Which organism matches the profile NLF; Oxidase positive; Alk/ALK; Motile?
• Which organism matches the following profile: short Gram-negative bacillus; oxidase variable; X factor positive; V factor negative; XV factor positive; porphyrins negative?
• Which genus is identified by UV fluorescence in combination with nitrate reduction?
• Which test result supports identification of EHEC O157:H7 aside from SMAC?
• Which specimen is collected in a White Top or Orange Top container?
• Which organism is commonly associated with nosocomial infections and produces a brick-red pigment on blood agar, with DNase activity producing a halo?
• Which components are typically included in a PCR master mix?
• Which antibiotic classes should be avoided for MRSA infections according to the guidelines?
• Which organism shows lecithinase activity on egg yolk agar?
• If an anaerobic specimen reveals terminal GPB spores and swarming on an ANO2 BAP plate, which organism is suspected?
• In some molecular workflows, the quantitation step is typically performed by:
• Which organism is associated with cystic fibrosis and demonstrates oxidase-positive, motile bacteria with glucose oxidation showing yellow in the O tube and green in the F tube?
• Which virus is listed with STI isolates as associated with AIDS?
• What is the purpose of using Selenite broth in stool cultures?
• Which organism is lactose fermenting, non-motile, and indole positive?
• Which genital specimen configuration is correct for gonorrhoeae testing?
• Which medium is specifically used for Neisseria species?
• Which organism is a Gram-positive bacillus with boxcar shape and positive for reverse CAMP test?
• In PCR, inadequate DNA extraction most often leads to which outcome?
• Which organism is associated with gonorrhea in STI isolates?
• Which antibiotic is listed as a treatment option for Bacteroides fragilis?
• Which organism is motile and yields an A/A glucose fermentation with VP positive?
• Which organism is a common cause of neonatal sepsis and meningitis and shows CAMP test positive with arrow-shaped hemolysis and blue Hippurate hydrolysis?
• Which medias are differential?
• Which organism is non-lactose fermenting, hydrogen sulfide positive, citrate positive, motile, PAD negative, and indole negative?
• In PCR testing for suspected vancomycin-resistant enterococci (VRE), which gene would you attempt to identify for Enterococcus faecium or Enterococcus faecalis?
• 42-year-old patient with white plaques in the mouth; Sabouraud dextrose agar yields white creamy colonies; Wet prep shows chlamydospores and pseudohyphae; Germ tube shows no constriction. Which organism is suspected?
• A 34-year-old patient has painful, red, and swollen skin lesions on the thigh. A wound swab is cultured. Gram stain shows Gram-positive cocci in clusters. Blood agar yields large golden beta-hemolytic colonies. MSA yellow; Catalase bubbling; Slide latex agglutination positive. Coagulase positive. DNAse halo around the organism. Which organism do you suspect?
• Gram-positive cocci in chains, beta-hemolytic, Lancefield group A, PYR positive. Which organism fits?
• Which antimicrobials are used to treat Streptococcus pyogenes infections, along with any relevant testing information?
• Immunocompromised patient with confusion and fever; CSF specimen cultured. Birdseed agar shows brown colonies; India ink shows encapsulated budding yeast with halo; Urea test is pink. Which organism is suspected?
• Which medium is commonly used for broad growth of gastrointestinal organisms in stool cultures?
• Which step in the molecular testing process is used to assess DNA integrity, presence of inhibitors, and purity?
• Which organism is a Gram-positive bacillus with boxcar morphology and a positive reverse CAMP test?
• What is the recommended antimicrobial for treating Streptococcus agalactiae (Group B Streptococcus) infections?
• Which organisms are classified as SPICE organisms?
• Which organism grows around the V factor but not the X factor on factor V/X enriched media and is implicated in respiratory infections?
• Which organism is non-lactose fermenting, oxidase negative, and shows DNAse positivity?
• Which agent is used to treat CRE (carbapenem-resistant Enterobacteriaceae) according to the material?
• Which organism is commonly isolated from wound infections?
• Which organism is commonly considered normal skin flora and commonly found on the surface of the skin?
• If you produce too light of an inoculum, what is the effect on MIC and KB zone sizes?
• Which of the following is NOT listed as a lower respiratory pathogen in the material?
• In antibiotic susceptibility testing (AST), if the growth medium is too thick, what is the most likely result?
• Which organism is a common stool pathogen?
• Beta-hemolytic streptococcus with Lancefield Group A. Which organism is this?
• Which organism is suggested by tumbling motility, umbrella pattern on motility, CAMP test positivity with S. aureus, bile esculin blackening, and VP red?
• Which organism is catalase positive and associated with Gram-positive bacteria in standard laboratory classification?
• Aspiration from a jaw abscess with Gram-positive branching filaments and sulfur granules; BAP shows white-gray molar-tooth-like colonies on day 3; Brucella agar also shows small similar colonies; Thioglycolate broth turbid; Penicillin is susceptible. Which organism is suspected?
• Which organism is associated with a painful genital ulcer and a Gram stain described as small Gram-negative coccobacilli in a school-of-fish arrangement?
• A 21-year-old female presents with vomiting, abdominal cramps, and water diarrhea after Chinese takeout; stool culture shows large beta-hemolytic colonies that are ground-glass-like on BAP; MAC shows no growth; Gram stain shows boxcar Gram-positive bacilli; Motility is Motile; Catalase bubbles. Which organism is suspected?
• Which organism is typically beta-hemolytic, catalase negative, PYR positive, and belongs to Lancefield group A?
• Which organism matches the following profile: short Gram-negative bacillus; oxidase variable; X factor negative; V factor negative; XV factor positive; porphyrins negative?
• Which organism requires both X and V factors for growth?
• Which organism is lactose fermenting, indole positive, urease negative, citrate negative, VP negative, and MUG positive?
• A 24-year-old patient with cystic fibrosis has a persistent cough; sputum culture shows Gram-negative bacilli; BAP grows after 3 days; MAC yields colorless colonies; Oxidase is positive with a purple reaction; Motility is evident; Glucose oxidation/oxidation tests: O tube yellow, F tube green; TSI is K/K. Which organism is suspected?
• Which organism matches the profile NLF; H2S; Indole negative; PAD positive; Urease positive?
• What media are selected for cerebrospinal fluid cultures to optimize growth of typical pathogens?
• Which is a common cause of false positives in PCR testing?
• Which media are used for urine cultures?
• Which antimicrobial class is ineffective against Mycoplasma pneumoniae due to its lack of a cell wall?
• What are the two different Chlamydia trachomatis bodies?
• What is the effect of overfilling a blood culture specimen?
• What is the preferred skin cleaning agent for blood culture collection or ethanol-based samples?
• Which organism is classically associated with boxcar Gram-positive rods and double-zone hemolysis on blood agar?
• Which organism is oxidase positive and does not ferment glucose (glucose neither), showing an alkaline reaction?
• Which medium is used to recover Bacteroides species?
• A 19-year-old patient presents with sudden high fever, severe headache, neck stiffness, nausea and confusion. There is a petechial rash on the lower limbs. A CSF sample was obtained and cultured. Gram stain shows Gram-negative cocci in kidney bean shape; Thayer-Martin yields smooth gray translucent colonies after 3 days. Which organism do you suspect?
• Stenotrophomonas maltophilia is treated with which agent?
• In nucleic acid amplification testing, what can cause a false positive?
• Which organism is a gram-negative cocci, oxidase positive, that ferments glucose, maltose, and sucrose but not lactose?
• Which organism is Gram-positive rods with terminal spores and slender filaments?
• Alpha-hemolytic streptococcus that is optochin susceptible and bile soluble. Which organism is this?
• Which organism yields green mold on SDA and is associated with pulmonary infections in an immunocompromised patient?
• A stool container is STAT and comes into the lab leaking. What do you do?
• Which organism matches the profile NLF; VP positive; Motile; A/A?
• In urinary culture interpretation, which scenario constitutes significant growth requiring workup?
• Which organism matches the profile NLF; VP negative; Non-motile; A/A?
• A Gram-positive bacillus with boxcar arrangement, catalase positive, and motile. Which organism is most likely?
• Which organism among the HACEK group is commonly associated with bite wounds and endocarditis?
• Which organism is characterized by Gram stain showing short GNB resembling coccobacilli, colorless colonies on MAC, CHOC enhanced growth, and non-motile?
• A 6-month-old infant presents with cough and fever; Regan-Lowe agar shows small, smooth, glistening colonies after 5 days; Bordet-Gengou agar shows similar colonies; BAP shows no growth; Oxidase is negative; Motility is non-motile. Which organism is suspected?
• Which description matches bacterial stool culture collection containers?
• Which organism demonstrates double-zone hemolysis on blood agar?
• Gram-positive cocci in clusters, catalase positive, novobiocin resistant. Which organism most likely?
• What is the correct protocol for collecting and transporting a Clostridium difficile stool specimen?
• Which organism is VP positive, motile, and lysine positive?
• Which organism is described as a short Gram-negative bacillus with oxidase variable and X factor negative?
• Which item is not typically considered part of the described molecular testing workflow?
• Bacteroides fragilis can be treated with which antibiotic?
• In a diabetic patient with a deep wound infection showing boxcar GPB, lecithinase activity, double-zone hemolysis, and reverse CAMP positive, which organism is most likely?
• Which medias are selective?
• Which antibiotic class should be avoided for ESBL-producing organisms among the following options?
• If the antibiotic disk is pushed deep into the agar during disk diffusion, what is the expected effect on the KB zone?
• If Shigella or Salmonella is suspected in a stool specimen, in what transport medium should it be sent?
• What antibiotic is used for urinary specimens only?
• UV fluorescence and nitrate reduction are characteristic features of which genus?
• Which organism ferments glucose but not maltose, sucrose, or lactose and is oxidase positive?
• Which organism is a member of the HACEK group and is commonly implicated in endocarditis, often listed alongside Eikenella corrodens and Capnocytophaga species?
• Which class of antibiotics is used to treat Mycoplasma pneumoniae?
• Which organism is oxidase positive and does not ferment glucose (glucose neither)?
• If the media is too thin, what happens in AST?
• How can you differentiate Candida krusei from Candida albicans?
• Which organism is non-lactose fermenting with hydrogen sulfide production, indole positive, PAD negative, and urea negative?
• Which organism is strongly associated with peptic ulcers and produces urease activity, contributing to its pathogenicity?
• Which list correctly identifies common urinary pathogens?
• A 35 year old patient presents with sudden watery diarrhea and severe dehydration. A stool sample is cultured. Gram stain shows comma-shaped Gram-negative rods; Thiosulfate citrate bile salt agar yields yellow colonies; oxidase is positive; motility is present; TSI shows A/A with no black precipitate; nitrate reduces to nitrite before zinc. Which organism do you suspect?
• If you use a 1.0 McFarland standard instead of 0.5, how will MIC and KB zone sizes be affected?
• Which of the following is NOT used for wound cultures?
• A 22-year-old patient has a high fever, abdominal pain, headache and constipation. A stool culture on Cary-Blair medium yields Gram-negative rods. HEK shows black colonies; TSI is K/A with black precipitate; Indole is red; VP yellow; Citrate blue; Phenylalanine yellow; Motility motile; Urea yellow. Which organism do you suspect?
• Gram-positive cocci in clusters, catalase positive, coagulase positive. Which organism best matches this profile?
• Which medias are enriched?
• Which media is used for Neisseria species recovery?
• If incubation of a Kirby-Bauer plate is delayed, what effect is observed?
• Which organism is beta-hemolytic on blood agar?
• Which organism is described as cocci-bacilli with oxidase positive and sick TSI?
• Germ tube test shows no constriction in an oral yeast culture; which organism is most likely?
• Pseudomonas aeruginosa is treated with which antibiotic from the list?
• Which organism is non-motile and indole negative?
• Which organism is a member of the HACEK group and is described as having a bleach-like odor and the ability to pit agar?
• Which organism is associated with syphilis?
• Which organism is described as GNB eyebrow and non-lactose fermenting?
• Which organism is non-lactose fermenting, oxidase negative, and shows oxidative metabolism (O/F test shows oxidation)?
• Which organism forms breadcrumb-like colonies on culture?
• Which organism is commonly considered normal skin flora or contaminants in wound cultures?
• Which organism is a gram-negative cocci that is oxidase positive but does not ferment glucose, maltose, sucrose, or lactose?
• What is the ideal treatment for chlamydia?
• A 62 year old patient presents with a persistent cough and rust-coloured sputum, fever, and shortness of breath. Sputum culture shows Gram-positive cocci in chains; the organism forms grey colonies with alpha hemolysis and a green halo on blood agar; catalase is negative; optochin shows growth; bile solubility is positive. Which organism is suspected, and what would you use for penicillin susceptibility testing?
• A 32-year-old patient presents after a hiking trip with a circular rash, low-grade fever; blood culture is flagged and Gram stain shows very thin, faint corkscrew-shaped spirochetes. ELISA and Western blot have been performed. Which organism is suspected?
• Which organism is alpha-hemolytic and optochin resistant with bile solubility negative?
• Which organism is described as SHORT GNB with oxidase variable, X negative, V positive, XV positive, and porphyrins positive?
• Which gene is targeted to identify methicillin resistant Staphylococcus aureus?
• A patient has a markedly elevated WBC count. What could cause a false positive blood culture?
• Which organism is non-lactose fermenting, hydrogen sulfide positive, PAD positive, urease positive, and indole positive?
• Which organism is lactose fermenting, indole positive, urease negative, citrate negative, VP negative, and MUG positive?
• A 58-year-old hospitalized patient presents with fever and respiratory deterioration despite broad-spectrum antibiotics. A sputum sample is cultured. Gram stain shows Gram-negative rods. Blood agar yields smooth gray colonies. MacConkey yields yellow colonies. Oxidase yellow; Catalase bubbling; Motility motile; DNase halo; Mannitol agar yellow. Which organism do you suspect and what antibiotic would you switch to?
• Which medium is used to detect Escherichia coli O157 in stool cultures?
• Which organism is commonly implicated in uncomplicated urinary tract infections in young women and is typically resistant to novobiocin?
• What is the effect of underfilling a blood culture specimen?
• Which organism is catalase positive and forms V forms among Gram-positive bacteria?
• If you use an expired antibiotic disk in a KB assay, what is the likely result?
• Which organism is NLF; Oxidase positive; Glucose neither; Alk/ALK?
• Which organism is a Gram-negative bacillus that is bile sensitive?
• Which organism is suspected in a pneumonia outbreak at a conference hotel and is confirmed by a urine antigen test?
• An elderly patient developed watery diarrhea after antibiotic use; which test would confirm Clostridium difficile infection?
• Which organism is Alpha-hemolytic, Optochin resistant, and Bile solubility negative?
• Which organism is a Gram-positive, branching rod that forms breadcrumb-like colonies and is partially acid-fast?
• On CHROMagar pink colonies, which VRE group is suspected?
• BBE medium growth is most characteristic of which Gram-negative genus?
• On CHROMagar blue colonies with arabinose negative, which Enterococcus species is suspected?
• Which organism is alpha-hemolytic and optochin resistant with bile solubility negative?
• A 59-year-old male with fever, abdominal pain, and foul wound drainage after abdominal surgery; a deep tissue biopsy is cultured. BAP shows smooth gray colonies; Bacteroides esculin agar shows black colonies; CHOC is non-growth; Gram stain shows small Gram-negative bacilli. Which organism is suspected?
• Which of the following is considered a priority set of specimens for microbiology?
• Which organism matches the profile NLF; Oxidase positive; Alk/ALK; Cetrimide positive?
• A 24-year-old patient presents with urethral discharge. Thayer-Martin medium yields smooth gray translucent colonies after 3 days; Gram stain shows Gram-negative diplococci in kidney-bean shape. Which organism is suspected?
• Gram-positive cocci in pairs, alpha-hemolytic, optochin susceptible, bile soluble. Which organism is this?
• A 78-year-old hospitalized patient with a catheter presents with fever; Gram-positive pleomorphic rods; BAP shows white pinpoint colonies; Catalase positive; Motility non-motile. What organism are you suspecting?
• Which organism is a Gram-negative bacillus with oxidase positive, bleach smell, and growth on Media PIT?
• For a methicillin-resistant Staphylococcus aureus (MRSA) infection, which agents are appropriate for treatment according to the guidelines?
• Which organism forms molar tooth colonies and branching Gram-positive bacilli?
• Which organism causes chancroid in STI isolates?
• Which organism is non-lactose fermenting, oxidase negative, nitrate positive, PAD positive, citrate positive, and indole positive?
• Which organism is non-lactose fermenting, oxidase negative, nitrate positive, VP positive, and motility positive?
• Which organism is a Gram-negative rod that is an indole-positive lactose fermenter and MUG positive in urine culture?
• What are the different grades for bacteria on a clinical Gram smear?
• Which organism is non-lactose fermenting, oxidase negative, and demonstrates DNAse positivity?
• Which organism is non-lactose fermenting, oxidase negative, nitrate positive, PAD positive, citrate negative, and indole positive?
• Which controls are typically included in PCR testing?
• Which antibiotic is used to treat Neisseria gonorrhoeae?
• Which organism is a curved, Gram-negative rod with gull-wing appearance, is microaerophilic, and shows positive hippurate hydrolysis?
• Which organism is a dimorphic fungus capable of systemic mycosis?
• Which criterion would lead to rejecting a sputum sample for culture?
• Which test differentiates Streptococcus pneumoniae from other alpha-hemolytic streptococci and is commonly used in identification?
• Which medium is specifically associated with selective recovery of Campylobacter from GI specimens?
• Which organism is lactose fermenting and hydrogen sulfide positive?
• In most Enterobacterales infections, including common urinary pathogens, which treatment approach is described as appropriate?
• Which organism is a Gram-negative bacillus that grows on BBE medium?
• A Gram-positive bacillus with long C shapes, catalase negative, alpha-hemolysis on blood agar. Which organism best fits this profile?
• Which organism is a Gram-positive bacillus with catalase positive, short bacilli, tumbling motility in wet preparation, umbrella-like motility on agar, and beta-hemolytic?
• Which scenario would cause the disk diffusion result to falsely indicate resistance?
• Which organism is a non-motile, gram-positive coryneform bacterium commonly associated with catheter-related bacteremia?
• Which organism is listed as an upper respiratory pathogen?
• A 6-year-old patient presents with fever, abdominal cramps, bloody diarrhea, and frequent small-volume stools. Stool culture shows Gram-negative rods; HEK yields green colonies; MAC yellow; Oxidase yellow; TSI K/A with no black precipitate; Motility non-motile; ONPG yellow; Ornithine purple. Which organism do you suspect?
• Which samples are considered irretrievable and will not be rejected for testing?
• Which organism is non-lactose fermenting, hydrogen sulfide positive, PAD positive, urease positive, and indole positive?
• Which organism is commonly identified by Thayer-Martin medium as smooth gray translucent colonies after 3 days and is a gram-negative diplococcus?
• For a Clostridium difficile stool collection, which transport statement is correct?
• Which organism forms Gram-positive branching filaments with molar-tooth colonies?
• Nitrofurantoin resistance is seen in which organism?
• Which media and conditions are used for blood cultures?
• A CHROMagar screening grows blue colonies. What do you do?
• During stool collection for C. difficile testing, what is the purpose of shaking the sample after adding fixative?
• Which statement best describes the correct blood culture collection procedure?
• How should ESBL-producing organisms be treated according to the guidelines?
• Which organism is detected by UV fluorescence and nitrate reduction?
• If you forget to add an antibiotic disk and then place it 30 minutes after incubation starts, what happens?
• A Gram-positive bacillus with boxcar arrangement, catalase positive, non-motile. Which organism is correct?
• Which organism is identified by yellow colonies on TCBS agar and oxidase positive?
• Which organism is described as Campylobacter, a GNB with oxidase positive and a bleach odor?
• Which regimen is recommended for methicillin-susceptible Streptococcus pneumoniae in severe cases, including meningitis considerations?
• A cystic fibrosis patient on mechanical ventilation has a chronic productive cough. A sputum sample shows Gram-negative rods. Culture yields large, flat irregular colonies with a metallic sheen at 42°C; growth on cetrimide is green; MAC is nonpigmenting; oxidase is positive; motility is present; nitrate is not reduced after zinc. Which organism do you suspect?
• Which sequence best represents the molecular testing workflow?
• If a urine sample has been collected and unpreserved for over 2 hours at room temperature, what should you do?
• Which antibiotic should be avoided in Pseudomonas aeruginosa infections according to the material?
• If you fail to adequately extract DNA, what would occur in PCR?
• Which organism shows Gram-negative diplococci that ferment both glucose and maltose in sugar utilization tests?
• A 42-year-old dairy farm worker presents with intermittent fever, night sweats, back pain, and weight loss over several weeks. Blood culture is performed in a biosafety cabinet due to aerosol risk. BAP shows small, smooth, convex colonies after 6 days; CHOC shows enhanced growth; Gram stain shows small GNB; Oxidase and Catalase tests are positive; Urea is pink within 2 hours. Which organism is suspected?
• Which organism is a Gram-positive bacillus that is catalase positive and displays V forms?
• Which organism is non-lactose fermenting and hydrogen sulfide positive, motile, with citrate positive and indole negative?
• Which organism is identified by red acid-fast rods on smear and breadcrumb-like colonies on Lowenstein-Jensen medium after several weeks of incubation?
• A 7-year-old unvaccinated child with sore throat and thick white pseudomembrane; throat culture shows Gram-positive pleomorphic rods; CTBA yields black-gray colonies; Catalase positive; Urea negative. What organism do you suspect and how do you identify it?
• Which medium would differentiate Gram-negative from Gram-positive organisms in wound cultures?
• A stool culture from a child with bloody diarrhea shows SMAC straw-colored colonies, motility, MUG fluorescence, and indole positive. Which organism is suspected?
• For methicillin-resistant Streptococcus pneumoniae, which agents are recommended for treatment, including meningitis considerations?